Analysis of the morphological features of VMH neurons filled with Lucifer yellow
| Oil (n = 4) | EB (n = 5) | |
|---|---|---|
| Soma area (μm2) | 107.10 ± 2.07 | 116.70 ± 10.44 |
| Dendrites/neuron | 2.9 ± 0.2 | 2.8 ± 0.2 |
| Primary dendrites/neuron | 2.1 ± 0.1 | 1.9 ± 0.1 |
| Branch points/neuron | 0.8 ± 0.2 | 0.9 ± 0.2 |
| Dendrite length (μm) | 113.50 ± 10.31 | 111.10 ± 15.08 |
Cells were filled iontophoretically with Lucifer yellow and visualized using confocal microscopy. Measurements were made on NIH Image 1.62 (mean ± SEM). EB, Estradiol benzoate. These measurements are consistent with previous Golgi studies (see Results and Discussion).