Table 1.

Parameters of cone-driven electroretinograms of WT, GRK1 −/−, and Arrestin −/− mice

ParameteramaxbmaxMUVUV/S̄M
UnitμVμV10−5 (photon μm2)−110−5(photon μm2)−1
WT (C57BL/6)12.6  ±  5.1 (21 ± 10)*100  ±  363.0 ± 2.0 17 ± 125.2  ±  1.8
n4   (9)* 11766
GRK1 −/−10.0  ±  5.197  ±  405.8 ± 1.920 ± 4.43.8  ±  1.6
n    1113555
Arrestin −/−9.1  ±  5.6129  ±  135.4 ± 3.114.5 ± 93.7  ±  1.3
n    14 6666
  • The top row identifies a measured or computed ERG parameter. amax is the saturating amplitude of the a-wave component of the ERG (Fig. 6), and bmax the saturating amplitudes of the gaussian-filtered b-wave, obtained under cone-isolation conditions (Fig. 4A).S̄M and S̄UVare the relative sensitivities of the cone-driven b-wave in the midwave and UV spectral regions, derived as follows. The peak amplitudes of gaussian-filtered cone b-wave responses were obtained in the linear region of the amplitude versus intensity function and were normalized by the amplitude of the saturating response (Fig. 4B);S̄M was calculated as the average of all data obtained with 492, 501, and 513 nm flashes, andS̄UV is the mean of all data obtained with 346, 356, and 361 nm flashes.S̄UV/S̄M is the ratio, determined separately for each animal. The errors given are SDs. *The observations in parentheses are taken from Lyubarsky et al. (1999) and were obtained in the presence of a steady background estimated to produce 6000 photoisomerizations rod−1sec−1 and shown to suppress at least 95% of the rod circulating current. To obviate a possible residual contribution from the rod a-wave that may have contaminated this previous estimate of the cone amax in some mice, the measurements of the cone a-waves of WT mice reported in this table (n = 4) were all obtained in a double-flash paradigm, which measures the cone response a few seconds after the rod a-wave is suppressed with a very intense flash (Lyubarsky et al., 1999) and which takes advantage of the very rapid recovery of the cone a-wave (Figs. 6,7).