Serotonin secretion from rat raphe neurons in primary culture
| [3H]Serotonin secretion (%) | ||
|---|---|---|
| No pretreatment | Pretreatment with TeNt | |
| Control | 13.0 ± 1.4 | 7.3 ± 0.47 |
| 50 mm K+ | 33.0 ± 1 | 12.3 ± 0.48 |
Raphe neurons (11 d in vitro) were treated with solvent or with 1 nm TeNt and further cultivated for another 2 d. [3H]Serotonin secretion was performed as described in Materials and Methods. Release is given as percentage of total [3H]serotonin present at the start of the experiment and represents the mean of three individual cultures ± SD. Note that TeNt pretreatment reduced basal as well as stimulated release.