Zinc exposure decreased NAD+, and this effect was reversed by addition of pyruvate or niacinamide
| Condition | NAD+ (nmol/plate) | NADH (nmol/plate) | Lactate (nmol/plate) |
|---|---|---|---|
| Control | 4.63 ± 0.31 | 0.58 ± 0.052 | 2020 ± 130 |
| Control + 4 mm pyruvate | 5.62 ± 0.59* | 0.20 ± 0.016* | 2930 ± 110* |
| Control + 1 mmniacinamide | 6.170 ± 0.36* | 0.66 ± 0.058 | 2650 ± 230* |
| 40 μm Zinc | 1.36 ± 0.16* | 0.83 ± 0.14 | 2470 ± 80* |
| 40 μm Zinc + 4 mm pyruvate | 4.86 ± 0.22# | 0.042 ± 0.027# | 3600 ± 140# |
| 40 μm Zinc + 1 mmniacinamide | 4.38 ± 0.40# | 0.89 ± 0.059 | 3270 ± 170# |
Neuronal cultures (6 × 106 cells per plate) were sham-washed (Control) or exposed to 40 μmZn2+ in the presence or absence of 4 mmpyruvate or 1 mm niacinamide for 4 hr, after which the cells were harvested and assayed for levels of NAD+, NADH, or extracellular lactate (results are pooled from three separate experiments; n = 5–8 cultures per condition). * signifies difference from sham-washed controls at p < 0.05 by two-way ANOVA, followed by a Bonferroni test.
signifies difference from Zn2+-treated cultures at p < 0.05.