DOI-induced increase in Fos immunoreactivity (Fos-IR) in hypothalamic nuclei and its blockade by pretreatment with the 5-HT2A antagonist MDL 100,907
| Treatment | Number of Fos-IR cells in hypothalamic nuclei | |
|---|---|---|
| Paraventricular | Supraoptic | |
| Saline–saline | 59.75 ± 23.5 | 8.5 ± 3.1 |
| Saline–DOI | 575.25 ± 60.81-160 | 26.0 ± 2.21-160 |
| MDL 100,907–saline | 165.25 ± 50.9 | 6.0 ± 0.7 |
| MDL 100,907–DOI | 117.0 ± 26.1 | 3.5 ± 2.2 |
The data represent the mean ± SEM of four rats per group. MDL 100,907 (0.1 mg/kg, s.c.) was injected 45 min before DOI (2.5 mg/kg, i.p.). The rats were killed 2 hr after the DOI injection.
↵F1-160 Significant difference from all other groups;p < 0.01 (two-way ANOVA and Newman–Keuls multiple range test).