Relative average intensity of nuclear anti-PER immunoreactivity in eyes of adult flies
| Time (hr) | Genotype | ||
|---|---|---|---|
| WT | dbtS | perS | |
| ZT13 | 0.8 (N/ W/S, 142) | 0.5 ( N/ W, 145) | 0.2 ( N/W, 79) |
| ZT15 | 1.2 ( W/S, 207) | 0.4 ( N/ W, 241) | 1.0 ( W, 91) |
| ZT16 | 1.3 ( W/ S, 222) | 0.7 (N/ W, 124) | |
| ZT17 | 1.6 ( W/ S, 117) | 0.8 ( N/ W, 75) | |
| ZT18 | 1.6 (W/ S, 369) | 1.3 ( W/ S, 435) | 0.9 (N/ W, 68) |
| ZT19 | 1.9 ( S, 160) | 1.2 (N/ W/ S, 77) | |
| ZT21 | 1.8 (W/ S, 375) | 1.7 (W/ S, 223) | 1.2 ( W/S, 65) |
| ZT1 | 1.8 (W/ S, 346) | 1.6 (W/ S, 174) | 0.9 (N/ W, 45) |
| ZT3 | 1.8 (W/ S, 264) | 1.7 (W/ S, 144) | |
| ZT5 | 1.4 ( W/ S, 149) | 1.2 (N/ W/ S, 143) | |
| ZT7 | 1.2 ( W/S, 233) | 0.9 (N/ W/S, 145) | |
| ZT11 | 0.8 (N/ W, 73) | 0.5 ( N/ W, 50) | |
Fly heads were collected, processed, and scored as described in Materials and Methods. Time is the time of collection in LD and is given in ZT, where ZT0 = lights on and ZT12 = lights off. A staining class present in 10–30% of the sections is indicated with a letter, whereas a staining class present in >30% of the sections is indicated with an underlined letter (N, no staining, score of 0; W, weak staining, score of 1; S, strong staining, score of 2). The number of sections scored is also indicated in parentheses. The average score (ranging from 0–2) is given for each condition. All 97 perO sections that were scored exhibited no staining (average score of 0).