Factors and drugs tested for their ability to alter migration from SVZ explants on PDL-plated dishes
| Factors tested in migration on PDL | Concentration |
|---|---|
| Bone morphogenic protein-7 | 50 ng/ml |
| Ciliary neurotrophic factor | 50 ng/ml |
| Brain-derived neurotrophic factor | 100 ng/ml |
| Neurotrophin-4 | 10, 100, 500 ng/ml |
| Epidermal growth factor | 10, 50 ng/ml |
| Endothelial cell growth factor | 20, 100 ng/ml |
| Keratinocyte growth factor | 10, 100 ng/ml |
| Glial-derived neurotrophic factor | 20 ng/ml |
| Granulocyte colony stimulating factor | 10 U/ml |
| Granulocyte-macrophage colony stimulating factor | 10 U/ml |
| Hepatocyte growth factor/scatter factor | 100 ng/ml |
| Leukocyte inhibitory factor | 10 ng/ml |
| Insulin-like growth factor-1 | 5, 50, 100 ng/ml |
| Platelet-derived growth factor B/B | 5, 10, 20, 100, 200 ng/ml |
| Retinoic acid | 100 nm |
| Stem cell factor | 50 ng/ml |
| Transforming growth factor-α | 5, 50 ng/ml |
| Transforming growth factor-β1 | 10 ng/ml |
| Tumor necrosis factor-α | 5 ng/ml |
| Leupeptin | 100 mm |
| High potassium (KCl) | 25 mm |
| Tetraethylammonium | 10 mm |
| 2-Amino-5-phosphono-valerate | 100 mm |
| Tetrodotoxin | 1 mm |
| ω-Conotoxin | 1 μm, 1 mm |
| Forskolin | 5 μm |
| Pertussis toxin | 100 ng/ml |
All treatments were tested for changes in migration in serum-containing and serum-free medium (1 and 2 DIV). None of the treatments led to significant changes in migration.