Distribution of autoradiographic silver grains and labeling densities in organelles of retinal ganglion cells 10 hr after intraocular injection of BDNF or NT-3 in chick embryos with or without the tyrosine kinase inhibitor K252a
| Organelle | BDNF | +K252a | NT-3 | +K252a | ||||
|---|---|---|---|---|---|---|---|---|
| % | LD | % | LD | % | LD | % | LD | |
| Plasma membrane | 20.0 | 3.70 | 19.8 | 3.67 | 6.7 | 1.24 | 19.0 | 3.52 |
| Vesicles | 9.9 | 1.68 | 12.0 | 2.03 | 9.8 | 1.66 | 6.6 | 1.12 |
| Cytoplasm | 13.7 | 0.74 | 17.7 | 0.96 | 20.2 | 1.10 | 13.4 | 0.73 |
| MVB | 6.8 | 6.80 | 1.5 | 1.50 | 3.5 | 3.50 | 3.6 | 3.60 |
| Lyso/endosome | 7.7 | 5.13 | 14.5 | 9.67 | 11.6 | 7.73 | 19.4 | 12.9 |
| Golgi | 9.8 | 2.39 | 2.1 | 0.51 | 18.7 | 4.56 | 3.1 | 0.76 |
| ER | 14.4 | 1.17 | 12.2 | 0.99 | 10.1 | 0.82 | 16.9 | 1.37 |
| Nucleus/membrane | 8.4 | 0.22 | 10.3 | 0.27 | 4.2 | 0.11 | 10.6 | 0.28 |
| Mitochondria | 2.0 | 0.26 | 3.7 | 4.9 | 4.6 | 0.60 | 4.3 | 0.57 |
| Unidentified | 7.2 | 1.36 | 6.7 | 1.26 | 8.6 | 1.62 | 3.0 | 0.57 |
| n | 162 | 197 | 119 | 166 | ||||
ER, Endoplasmic reticulum; LD, labeling density; MVB, multivesicular body. Nucleus and nuclear membrane were evaluated separately but always showed random distribution and were grouped together. LDs of >3.3 are indicated in bold.