Perifornical region cell counts, sleep–wake behavior,Tb, and LMA of rats killed at different times under LD or DD conditions
| LD | DD | |||||||
|---|---|---|---|---|---|---|---|---|
| ZT 3 | ZT 9 | ZT 15 | ZT 21 | Kruskal–Wallis p value | CT 3 | CT 15 | Mann–Whitney p value | |
| n | 6 | 7 | 6 | 7 | 6 | 6 | ||
| Fos-IR neurons | 31 ± 4 | 44 ± 8 | 80 ± 7 | 72 ± 5 | 0.001 | 25 ± 5 | 60 ± 6 | 0.007 |
| Orexin-IR neurons | 79 ± 5 | 80 ± 3 | 71 ± 5 | 74 ± 3 | N.S. | 83 ± 4 | 80 ± 2 | N.S. |
| Double-labeled neurons | 15 ± 2 | 19 ± 5 | 32 ± 5 | 36 ± 2 | 0.009 | 7 ± 3 | 26 ± 3 | 0.007 |
| Non-orexin-IR, Fos-IR neurons | 16 ± 4 | 25 ± 4 | 47 ± 5 | 36 ± 3 | 0.001 | 18 ± 4 | 34 ± 3 | 0.03 |
| % Wake | 38.1 ± 12.9 | 46.6 ± 4.0 | 71.7 ± 5.4 | 72.6 ± 7.4 | 0.008 | 38.3 ± 8.4 | 79.9 ± 3.5 | 0.007 |
| % NREM sleep | 53.1 ± 10.7 | 46.6 ± 3.7 | 23.3 ± 4.0 | 25 ± 6.7 | 0.01 | 52.6 ± 6.8 | 18.6 ± 3.0 | 0.007 |
| % REM sleep | 8.8 ± 3.6 | 6.8 ± 1.4 | 5.0 ± 1.6 | 2.4 ± 0.9 | N.S. | 9.2 ± 3.2 | 1.5 ± 0.9 | 0.05 |
| Tb (°C) | 37.2 ± 0.1 | 37.0 ± 0.1 | 37.9 ± 0.1 | 37.8 ± 0.1 | 0.001 | 37.1 ± 0.1 | 37.7 ± 0.1 | 0.01 |
| Change in Tb (°C) | −0.1 ± 0 | 0.3 ± 0.1 | −0.1 ± 0 | −0.2 ± 0.2 | 0.03 | 0.0 ± 0.1 | 0.0 ± 0.1 | N.S. |
| LMA | 0.3 ± 0.1 | 0.6 ± 0.1 | 1.5 ± 0.2 | 1.4 ± 0.1 | 0.0007 | 0.6 ± 0.1 | 1.7 ± 0.2 | 0.01 |
Behavior over the 2 hr before death was used to calculate the physiologic measures. Values are means ± SE. N.S., Not significant.