Sequences of RT-PCR primers, product sizes, and enzymes used for restriction analyses
| Primer sequence | Product size (bp) | Enzyme (site location, bp) | Accession number | |
|---|---|---|---|---|
| TRα1 | (For) 5′-TTGGAAACAGAGGCGAAAAT-3′ | 788 | PstI (408) | M18028 |
| (Rev) 5′-GGGAGGAAGGAGAGAAGAGA-3′ | ||||
| TRα2vI/TRα2vII | (For) 5′-TGACCCTGAGAGCGACACCC-3′ | 598/481 | Bg11 (366/none) | M31174 |
| (Rev) 5′-CTCACTGCTGTCGTCTTCCC-3′ | EcoRI (552/435) | |||
| TRβ1 | (For) 5′-TTCCCTCTCCTTAGTCTGCT-3′ | 710 | BamHI (322) | JO3819 |
| (Rev) 5′-GCCTCTTCTCACGGTTCTCT-3′ | ||||
| TRβ2 | (For) 5′-ACCCCAAGACCTCCGTTTTT-3′ | 785 | SfiI (430) | M25071 |
| (Rev) 5′-TCGCCTCCGCACTCACACAA-3′ | ||||
| GAPDH | (For) 5′-TATCGGACGCCTGGTTACCA-3′ | 875 | BglI (440) | X02231 |
| (Rev) 5′-CATTGAGAGCAATGCCAGCC-3′ |
Forward and reverse primers are indicated as (For) and (Rev), respectively. Restriction sites are located in amplified products with respect to the 5′ end of the sense strand. TRα2vI and TRα2vII PCR products were generated with the same primers. Sequences of TRα2vI- and TRα2vII-amplified products differed by the 117 bp deletion (313–429 bp) in the latter as determined by Lazar et al. (1988) andMitsuhashi et al. (1988b).