Loss of the neuroprotective effect of 17-β estradiol on AF64A (40 μm)-induced neuronal cell death in hippocampal and septal cell cultures after pretreatment with tamoxifen, ICI 182,780, LY 294002, or cycloheximide
| Cell culture | AF64A + vehicle | AF64A + antagonist or inhibitor | AF64A + estradiol 0.1 μm | AF64A + estradiol + antagonist or inhibitor | |
|---|---|---|---|---|---|
| Tamoxifen 1 μm | Hippocampal | 41.9 ± 4.7 | 36.5 ± 4.1 | 21.0 ± 3.32-a | 44.8 ± 6.22-b |
| ICI 182,780 1 μm | Hippocampal | 65.5 ± 4.8 | 66.6 ± 3.8 | 31.8 ± 3.02-c | 68.7 ± 3.52-d |
| Septal | 49.4 ± 5.5 | 43.9 ± 1.9 | 24.9 ± 3.42-a | 50.7 ± 2.62-d | |
| LY 294002 5 μm | Hippocampal | 65.5 ± 4.8 | 61.3 ± 2.5 | 31.8 ± 3.02-c | 57.1 ± 5.12-d |
| Septal | 49.4 ± 5.5 | 49.2 ± 4.3 | 24.9 ± 3.42-a | 46.5 ± 6.12-b | |
| Cycloheximide 500 ng/ml | Hippocampal | 53.6 ± 4.2 | 54.5 ± 5.2 | 31.4 ± 3.42-a | 52.5 ± 4.22-b |
The values represent the AF64A-induced increase in LDH-release (Δ U/ml medium). The basal LDH-release in the corresponding control cultures was 43.3 ± 4.9 (hippocampal) and 41.7 ± 3.2 U/ml medium (septal). n = 4–9.
↵F2-a p < 0.05 versus AF64A + vehicle.
↵F2-b p < 0.05 versus AF64A + estradiol.
↵F2-c p < 0.001 versus AF64A + vehicle.
↵F2-d p < 0.001 versus AF64A + estradiol.