Effects of pharmacological agents on amplitude, time course, and frequency of mIPSCs in PNs
| Nodularin | KN62 | KT5720 | FK506 | |||||
|---|---|---|---|---|---|---|---|---|
| Before | 15 min | Before | 15 min | Before | 15 min | Control | FK506 | |
| Amplitude (pA) | 13.9 ± 3.3 | 13.2 ± 3.9 | 11.7 ± 2.2 | 11.2 ± 2.0 | 10.4 ± 2.3 | 10.5 ± 3.2 | 15.2 ± 3.0 | 14.1 ± 2.6 |
| 10–90% rise time (msec) | 3.4 ± 0.5 | 3.0 ± 0.5 | 3.8 ± 0.6 | 3.9 ± 0.4 | 3.1 ± 0.2 | 3.0 ± 0.5 | 3.4 ± 0.8 | 3.4 ± 0.5 |
| Half-height width (msec) | 16.2 ± 2.6 | 17.3 ± 3.1 | 20.0 ± 1.9 | 21.1 ± 2.4 | 17.2 ± 0.8 | 18.9 ± 1.7 | 16.7 ± 3.1 | 17.9 ± 2.4 |
| Frequency (Hz) | 9.7 ± 2.2 | 8.8 ± 3.4 | 12.0 ± 3.2 | 12.6 ± 8.3 | 9.4 ± 2.0 | 13.4 ± 9.0 | 10.7 ± 4.2 | 12.1 ± 4.2 |
Values before and 15 min after the application of nodularin (n = 5), KN62 (n = 5), and KT5720 (n = 5) are shown. As for FK506, values from PNs with no treatment (control; n = 13) and those from PNs to which FK506 was introduced through a patch pipette (FK506;n = 11) are shown. Data are presented as mean ± SD.