Clinical rating scale used to quantify 3NP-induced motor symptoms in rats
| Score | Maximum score (near-death rat) | |
|---|---|---|
| Recumbency | ||
| All four legs active and four paws facing the floor | 0 | — |
| Two hind legs unable to sustain walk and body weight but forepaw able to keep upright position balance | 1 | — |
| Animal lying on its side, falling when put on its feet, showing rapid breathing, uncoordinated movements and chewing, almost complete paralysis | 2 | 2 |
| Hind legs | ||
| Normal, active gait | 0 | — |
| Presence of intermittent dystonia in one leg | 1 | — |
| Intermittent dystonia in two hind legs or permanent dystonia in one hind leg | 2 | — |
| Permanent dystonia in two hind legs | 3 | 3 |
| Gait | ||
| Normal gait | 0 | — |
| Presence of wobbling, uncoordinated, jumpy gait | 1 | 1 |
| Balance | ||
| Ability to keep balance for 10 sec on a 5 × 10 cm platform (15 cm height) | 0 | — |
| Inability to keep balance | 1 | 1 |
| Grasping | ||
| Ability to grasp a cage grid with at least one forepaw for 10 sec | 0 | — |
| Inability to grasp | 1 | 1 |
| Total | 8 |