Time | Genes | Function | METH/control | METH + WIN/control | WIN/control |
---|---|---|---|---|---|
3 hr | Adenylyl cyclase III | Signal transduction | ↓ 2.22 (2.04–2.50)1-a | ↓ 1.37 (1.23–1.54) | ↓ 1.08 (1.02–1.14) |
SLC10A1 | Ion transportation | ↓ 2.33 (2.13–2.63)1-a | ↓ 1.43 (1.30–1.56) | ↓ 1.11 (1.10–1.12) | |
SLC9A3R1 | Ion transportation | ↓ 2.13 (2.00–2.27)1-a | ↓ 1.45 (1.35–1.55) | ↓ 1.08 (1.04–1.11) | |
12 hr | COX1 | ATP generation (major player) | ↑ 2.40 (2.15–2.70)1-b | ↑ 1.68 (1.52–1.78) | ↑ 1.47 (1.36–1.58) |
NADH2 | ATP generation | ↓ 2.13 (2.04–2.22)1-b | ↓ 1.27 (1.27–1.28) | ↓ 1.02 (1.01–1.03) | |
NDR3 | Cell differentiation | ↓ 2.02 (1.96–2.08)1-b | ↓ 1.33 (1.30–1.37) | ↓ 1.09 (1.05–1.14) | |
24 hr | COXI | ATP generation (major player) | ↑ 2.62 (2.45–3.01)1-b | ↑ 1.35 (1.30–1.40) | ↑ 1.90 (1.78–1.98) |
Phosphoglycerate mutase B | Glycolysis for ATP | ↓ 2.17 (2.08–2.27)1-a | ↑ 1.06 (1.02–1.10) | ↓ 1.33 (1.25–1.43) | |
Tau | Neurodegeneration | ↓ 2.17 (2.04–2.38)1-a | ↓ 1.10 (1.07–1.12) | ↓ 1.22 (1.20–1.24) | |
NDR3 | Cell differentiation | ↓ 2.00 (1.90–2.10)1-b | ↓ 1.12 (1.09–1.22) | ↓ 1.33 (1.25–1.41) |
Microarray hybridizations were performed on both DA and neuronal chips containing ∼1600 genes, as described in Material and Methods. Data shown represent average fold changes compared with saline control, with range of fold changes included in the parentheses. Only those genes upregulated or downregulated by at least a factor of 2 are shown.
↵F1-a Indicates that at least two best sets of the microarray data were used to calculate the mean fold change for that gene, with more than twofold change after METH and <1.5-fold change after METH + WIN and WIN treatments. The main function of each gene is listed. SLC10A1, Solute carrier family 10 (sodium/bile acid cotransporter) member 1; SLC9A3R1, solute carrier family 9 (sodium/hydrogen exchanger) isoform 3 regulatory factor 1; COX1, cytochrome c oxidase subunit 1; NADH2, NADH ubiquinone oxidoreductase chain 2; NDR3, N-myc downstream regulated gene 3; Tau, tau microtubule-associated protein.
↵F1-b Indicates that at least four best sets of microarray results were used to calculate the mean fold change for that gene and that there was statistically difference in fold change between METH and METH + WIN35,428 or WIN35,428 alone, using one-way ANOVA (Duncan test) at a level of p < 0.05.