Summary of quantitative analysis of the longest neurites from hippocampal neuron cultures on TN-C Fc recombinant protein substrates
| Condition | Substrate | N | n | Neurite length, mean ± SEM (μm) | Minimum (μm) | Maximum (μm) | Neurites >100 μm (%) | ANOVA test* |
|---|---|---|---|---|---|---|---|---|
| Neurite outgrowth assays on TNfnIII-Fc recombinant proteins | ||||||||
| Control | Porn | 5 | 811 | 39.2 ± 2.4 | 10 | 133 | 0.95 | NS |
| LN-1 | 4 | 654 | 67.9 ± 11.8 | 14 | 240 | 14.2 | +++ | |
| TN-C | 3 | 515 | 58.9 ± 9.4 | 10 | 167 | 9.59 | +++ | |
| Fc | 5 | 751 | 41.7 ± 3.7 | 10 | 172 | 1.28 | NS | |
| TNfnA1A2-Fc | 5 | 691 | 41.1 ± 4.5 | 11 | 156 | 1.14 | NS | |
| TNfnA1D-Fc | 5 | 752 | 39.9 ± 3.4 | 12 | 139 | 0.11 | NS | |
| TNfnBD-Fc | 5 | 826 | 58.6 ± 11.2 | 11 | 191 | 7.89 | +++ | |
| TNfnCD-Fc | 5 | 787 | 45.1 ± 7.1 | 11 | 147 | 2.47 | + | |
| TNfnD6-Fc | 5 | 801 | 56.1 ± 10.8 | 11 | 242 | 6.69 | +++ | |
| TNfn78-Fc | 5 | 835 | 39.9 ± 4.1 | 11 | 154 | 0.63 | NS | |
| Kaf14 (30 μg/ml) | Porn | 4 | 583 | 33.8 ± 1.6 | 10 | 125 | 0.12 | NS |
| LN-1 | 4 | 637 | 63.6 ± 5.9 | 10 | 245 | 11.2 | +++ | |
| TN-C | 3 | 247 | 36.3 ± 2.2 | 11 | 131 | 0.49 | NS | |
| Fc | 4 | 624 | 36.0 ± 4.6 | 10 | 125 | 0.66 | NS | |
| TNfnA1A2-Fc | 4 | 566 | 34.3 ± 3.4 | 10 | 111 | 0.48 | NS | |
| TNfnA1D-Fc | 4 | 600 | 34.2 ± 4.0 | 10 | 118 | 0.36 | NS | |
| TNfnBD-Fc | 4 | 553 | 35.8 ± 2.2 | 10 | 112 | 0.26 | NS | |
| TNfnCD-Fc | 4 | 620 | 35.2 ± 3.8 | 11 | 123 | 0.34 | NS | |
| TNfnD6-Fc | 4 | 594 | 36.4 ± 3.1 | 10 | 121 | 0.23 | NS | |
| TNfn78-Fc | 4 | 483 | 34.9 ± 1.6 | 10 | 91 | 0 | NS | |
| Neurite outgrowth anti-F3 antibody perturbation assays on TNfnIII-Fc recombinant proteins | ||||||||
| pAbF3 (30 μg/ml) | Porn | 5 | 779 | 37.9 ± 4.9 | 10 | 125 | 0.86 | NS |
| LN-1 | 4 | 654 | 71.2 ± 8.8 | 12 | 275 | 15.1 | +++ | |
| TN-C | 3 | 569 | 53.9 ± 7.3 | 10 | 243 | 4.82 | +++ | |
| Fc | 5 | 729 | 39.9 ± 6.6 | 11 | 152 | 1.41 | NS | |
| TNfnA1A2-Fc | 5 | 809 | 39.7 ± 3.4 | 10 | 132 | 0.82 | NS | |
| TNfnA1D-Fc | 5 | 774 | 39.4 ± 4.7 | 12 | 113 | 0.97 | NS | |
| TNfnBD-Fc | 5 | 825 | 42.8 ± 6.2 | 11 | 161 | 1.21 | + | |
| TNfnCD-Fc | 5 | 811 | 41.9 ± 5.9 | 10 | 135 | 1.16 | + | |
| TNfnD6-Fc | 5 | 834 | 53.5 ± 11.1 | 11 | 184 | 6.57 | +++ | |
| TNfn78-Fc | 5 | 747 | 38.4 ± 4.3 | 10 | 120 | 0.66 | NS | |
| pAbF3 + peptide (30 + 10 μg/ml) | Porn | 4 | 636 | 39.9 ± 3.1 | 13 | 145 | 0.55 | NS |
| LN-1 | 4 | 638 | 73.3 ± 13.3 | 10 | 346 | 20 | +++ | |
| TN-C | 3 | 488 | 57.3 ± 2.5 | 10 | 201 | 8.02 | +++ | |
| Fc | 4 | 682 | 41.1 ± 6.5 | 11 | 140 | 1.4 | NS | |
| TNfnA1A2-Fc | 4 | 595 | 43.1 ± 4.8 | 11 | 128 | 0.85 | NS | |
| TNfnA1D-Fc | 4 | 635 | 40.4 ± 1.8 | 10 | 124 | 0.36 | NS | |
| TNfnBD-Fc | 4 | 675 | 57.8 ± 5.4 | 10 | 163 | 6.9 | +++ | |
| TNfnCD-Fc | 4 | 601 | 44.8 ± 7.7 | 10 | 167 | 2.35 | + | |
| TNfnD6-Fc | 4 | 638 | 56.4 ± 7.1 | 10 | 208 | 7.25 | +++ | |
| TNfn78-Fc | 4 | 628 | 40.5 ± 4.6 | 10 | 125 | 0.99 | NS | |
N, Number of independent experiments; n, number of total measured neurites.
↵* ANOVA test with risk, p = 0.05. Significance: +++, p < 0.001; ++, p < 0.003; +, p < 0.05; NS, not significant,p > 0.05.