0.2 mmNeomycin | 0.5 mm Neomycin | 1 mm Neomycin | ||||
---|---|---|---|---|---|---|
0.1% DMSO | 50 μmBAF | 0.1% DMSO | 50 μm BAF | 0.1% DMSO | 50 μm BAF | |
Extrastriolar | 68.5 ± 2.8 | 101.2 ± 4.6 | 60.2 ± 4.1 | 94.7 ± 5.1 | 50.9 ± 3.4 | 89.2 ± 4.0 |
Striolar | 40.0 ± 1.4 | 59.5 ± 2.4 | 23.5 ± 2.4 | 38.4 ± 2.8 | 18.5 ± 1.3 | 31.3 ± 1.8 |
Utricles were cultured for 24 hr with 0.2, 0.5, or 1 mm neomycin and 0.1% DMSO or 50 μm BAF. Calretinin+ cells were quantified in 10,000 μm2 regions of both the extrastriolar (six regions/organ) and striolar (four regions/organ) areas. Results are the mean ± SEM for three experiments from 10–13 organs.