Effects of SR140333, SR48968, or SR142801 on the NMDA-evoked release of [3H]-ACh in matrix and striosomes after chronic 6-OH-DA-induced dopaminergic denervation of the striatum
| Matrix | Striosomes | ||
|---|---|---|---|
| Unlesioned (%) | 6-OHDA-lesioned (%) | 6-OHDA-lesioned (%) | |
| NMDA/d-serine | 204 ± 11 | 279 ± 29 | 290 ± 7 |
| +SR140333 0.1 μm | 179 ± 13 | 180 ± 10* | 297 ± 18 |
| +SR48968 0.1 μm | 279 ± 8* | 201 ± 3* | 281 ± 17 |
| +SR142801 0.1 μm | 267 ± 30* | 171 ± 22* | 281 ± 16 |
One month after the 6-OH-DA injection into the fields of Forel, superfusion experiments and expression of data were performed as described in Materials and Methods and in Table 1 legend. NMDA 1 mm + 10 μmd-serine was applied for 2 min, 70 min after the beginning of the washing period. When used, SR140333, SR48968, or SR142801 (0.1 μm for each) was added to the ACSF from the start of the washing period up to the end of the experiment. Results are the means ± SEM of data obtained in four to six experiments.
↵* p < 0.05 when NMDA responses obtained in the presence of NK1 (SR140333), NK2 (SR48968), or NK3 (SR142801) receptor antagonists were compared with the respective effect of NMDA in the absence of the tachykinin antagonists in the matrix.