Effects of JB-1 on 3H-prazosin binding affinity
| KD (nm ± SEM) | ||||
|---|---|---|---|---|
| OVX | EB | |||
| Saline | JB-1 | Saline | JB-1 | |
| POA | 0.45 ± 0.08 | 0.70 ± 0.11 | 0.66 ± 0.11 | 0.82 ± 0.17 |
| HYP | 0.48 ± 0.10 | 0.54 ± 0.10 | 0.78 ± 0.15 | 0.64 ± 0.10 |
OVX female rats were primed with vehicle (OVX), or with EB (2 μg, 24 and 48 hr before killing). Multiple infusions of saline or JB-1 were given 1 hr before the first EB or vehicle injection and every 12 hr thereafter. KD values were obtained from Scatchard analysis of 3H-prazosin binding. Each value represents the mean of four independent replications.Bmax values are shown in Figure1A.