Regional distribution of Nogo-A, -B, -C protein expression in the adult rat
| Location | Nogo-A1-a | Nogo-A/B1-b | Nogo-C1-c |
|---|---|---|---|
| Spinal cord | |||
| White matter | +++1-d | +++1-d | −1-d |
| Oligodendrocyte cell bodies | +++1-d,1-e | +++1-d,1-e | (+)1-e,1-f |
| Astrocytes | − | − | − |
| Motor neurons | +1-e | +1-e | − |
| Interneurons | +1-e | +1-e | − |
| Cerebral cortex | |||
| Layer I | −1-d,1-e | −1-d,1-e | − |
| Layer II/III | ++1-d,1-e | ++1-d,1-e | (+)1-e,1-f |
| Layer IV | +1-d,1-e | +1-d,1-e | (+)1-e,1-f |
| Layer V/VI | ++1-d,1-e | ++1-d,1-e | (+)1-e,1-f |
| Hippocampus | |||
| CA1–CA4 | ++1-d,1-e | ++1-d,1-e | (+)1-e,1-f |
| Dentate gyrus | +1-d,1-e | +1-d,1-e | (+)1-e,1-f |
| Habenula | +++1-d,1-e | +++1-d,1-e | (+)1-e,1-f |
| Cerebellum | |||
| Granule cells | −1-g | −1-g | − |
| Purkinje cells | +1-d,1-e | +1-d,1-e | +1-e,1-f |
| Molecular layer | +1-d,1-e | +1-d,1-e | +1-e,1-f |
| Deep cerebellar nuclei | +++1-d,1-e | +++1-d,1-e | −1-e,1-f |
| Retina | |||
| Retinal ganglion cells | ++1-d,1-e | ++1-d,1-e | − |
| Inner and outer nuclear layer | ++1-d,1-e | ++1-d,1-e | − |
| Peripheral ganglia | |||
| Dorsal root ganglia | ++1-d,1-e | ++1-d,1-e | − |
Semiquantitative analysis of the Nogo expression in the nervous system as found in immunohistochemistry on tissue sections. −, Undetectable; (+), very weak; +, present; ++, strong; +++, very strong.
↵F1-a AS 472.
↵F1-b AS Bruna or AS Bianca.
↵F1-c AS 818.
↵F1-d Fresh frozen sections post-treated with ethanol and acetic acid or EGTA-ethanol and acetic acid.
↵F1-e Fresh frozen sections post-treated with methanol or methanol-DMSO.
↵F1-f Fresh frozen sections post-treated with 0.1% paraformaldehyde for immunohistochemical visualization of the three Nogo isoforms in different tissues.
↵F1-g Granule cell layer of the cerebellum shows weak unspecific staining (negative in in situhybridization and histoblot).