Growth-inhibitory activities of NG2 fusion proteins
| Condition | Neurite length (μm) | Inhibition (%) | EC50(nm) | Collapsed growth cones (%) | EC50(nm) |
|---|---|---|---|---|---|
| Control | 107.8 ± 2.8 | 31.2 ± 0.6 | |||
| ECD | 58.5 ± 2.1 | 46.3 | 3.5 | 49.8 ± 1.5 | 1.8 |
| D1-Fc | 67.4 ± 2.6 | 44.7 | 4.5 | 48.9 ± 1.2 | 3.1 |
| D2-Fc | 67.7 ± 3.8 | 39.2 | 9 | 48.5 ± 2.3 | 3.5 |
| D2-Fc + C'ase | 84.1 ± 4.5* | 24.4 | 22 | 34.4 ± 1.1* | >20 |
| D1,2-Fc | 49.6 ± 2.0 | 52.6 | 3 | 49 ± 2.3 | 2.5 |
| D1,2-Fc + C'ase | 50.6 ± 2.2 | 51.7 | 3.5 | 51.1 ± 0.1 | 2.4 |
| D3-Fc | 65.6 ± 2.6 | 43.4 | 6 | 50.3 ± 1.4 | 2.4 |
Data shown are the mean ± SEM determined from two to seven experiments for each data set. Percentage of inhibition and percentage of collapse were determined using the fusion proteins at 20 nm. The EC50 values were calculated from the data shown in Figures 3 and 4. C'ase indicates that the proteins were treated with chondroitinase ABC as described in Materials and Methods.
↵* Values were not significantly different from control values (p > 0.05; ANOVA and Scheffé'spost hoc test).