Effect of PLC-inhibitor U73122 and its inactive isomer U73343 on muscarine responses
| gM suppression by 10 μm muscarine | gM suppression by 10 μm muscarine in presence of inhibitor | |
|---|---|---|
| U73122 (10 μm) (n = 6) | 80.8 ± 4.2% | 10.2 ± 2.8% (p < 0.0001) |
| 13.6 ± 3.7% (p < 0.0002) p = 0.5 | ||
| U73343 (10 μm) (n = 5) | 81.7 ± 5.4% | vs 10 μm U73122 |
| U73122 (3 μm) (n = 7) | 70.3 ± 3.9% | 11.6 ± 3.0% (p < 0.0001) |
| 24.7 ± 5.4% (p < 0.005) p < 0.05 | ||
| U73343 (3 μm) (n = 5) | 84.0 ± 2.6% | vs 10 μm U73122 |
Paired t tests were used to compare gM suppression before and after the application of inhibitors in each cell studied. Unpaired t tests were used to compare differences between gM suppression in groups of cells in the presence or absence of inhibitors. Whole-cell recordings were used in all cases.