Characteristics of spontaneous EPSCs recorded from CA3 pyramidal cells and interneurons before bicuculline application was started (before bicuculline) or 3 hr after beginning its washout (after bicuculline)
| Treatment | CA3 pyramidal cells | CA3 interneurons | ||||||
|---|---|---|---|---|---|---|---|---|
| Amplitude (pA) | Rise time (msec) | Decay time (msec) | n | Amplitude (pA) | Rise time (msec) | Decay time (msec) | n | |
| Wild type (WT) | ||||||||
| Before bicuculline | 25 ± 2 | 2.2 ± 0.3 | 7.6 ± 1.4 | 30 | 40 ± 6 | 1.3 ± 0.2 | 3.3 ± 0.2 | 30 |
| After bicuculline | *39 ± 2 | 2.1 ± 0.4 | 6.7 ± 1.1 | 21 | *21 ± 3 | 1.2 ± 0.4 | 4.3 ± 0.9 | 19 |
| mGluR5 K0 | ||||||||
| Before bicuculline | 26 ± 4 | 2.1 ± 0.3 | 6.5 ± 1.3 | 15 | 34 ± 3 | 1.5 ± 0.2 | 4.5 ± 0.9 | 18 |
| After bicuculline | 23 ± 3 | 2.3 ± 0.9 | 6.8 ± 0.9 | 13 | 36 ± 3 | 1.1 ± 0.5 | 4 ± 0.4 | 17 |
| WT + MPEP | ||||||||
| Before bicuculline | 27 ± 5 | 2.2 ± 0.4 | 6.7 ± 1.3 | 17 | 39 ± 3 | 1.3 ± 0.3 | 3.4 ± 0.4 | 15 |
| After bicuculline | 26 ± 3 | 2.4 ± 0.5 | 6.5 ± 1.4 | 15 | 38 ± 2 | 1.4 ± 0.2 | 3.5 ± 0.5 | 11 |
| WT + AP-5 | ||||||||
| Before bicuculline | 27 ± 5 | 2.2 ± 0.8 | 5.2 ± 1.4 | 14 | 37 ± 4 | 1.4 ± 0.6 | 2.8 ± 0.5 | 13 |
| After bicuculline | 28 ± 3 | 2.4 ± 0.7 | 4.7 ± 1.1 | 12 | 38 ± 3 | 1.3 ± 0.9 | 2.5 ± 0.4 | 10 |
Incubations with MPEP and AP-5 were started 30 min before bicuculline application until bicuculline washout. Mean values ± SEM are given. Asterisks indicate significant differences (ANOVA; *p < 0.05) compared with wild type before bicuculline. n indicates number of cells.