Rise and decay time were measured between 10 and 90% of the peak value. Half-width is the time between 50% of the peak value on the rising phase and 50% of the peak value on the falling phase. In Wt and α6−/− animals, decay time was not accurately measured because, in many cases, current did not come back to baseline levels before the end of the registering period (1.5 sec). In α4−/− animals after αCtxMII inhibition, kinetic parameters were calculated only when the amplitude of residual current was sufficient to make a precise measure (>40pA). ^*p<0.05, ^**p<0.01 compared with Wt. °p<0.05 compared with α4−/−α6−/−; Student's t test. αCtxMII inhibition is expressed as a percentage of basal response before antagonist application. Maximal amplitude and current kinetics were measured on three successive responses before and after (6 min) αCtxMII (100 nM) bath perfusion. Because αCtxMII inhibitory effect was small and irreversible (even after 10 min washout period), only neurons exhibiting a stable response to ACh application were kept in the analysis. †p<0.001 compared with αCtxMII-free condition; paired Student's t test.