Numbers of myelinated and unmyelinated axons in control and rhGDNF-treated rats
| Animal 1 | Animal 2 | Animal 3 | Animal 4 | |
|---|---|---|---|---|
| Vehicle-treated | 10,804 (18,465) | 9,089 (13,075) | 11,191 (18,259) | 9,888 (18,282) |
| rhGDNF-treated | 15,718 (18,276) | 10,473 (16,738) | 15,717 (17,511) | 13,246 (15,738) |
The numbers of myelinated and unmyelinated (in parentheses) axons per nerve in vehicle control or rhGDNF-injected animals at 4 weeks are shown. There was an increase in the number of myelinated axons in rhGDNF-treated rats (p = 0.038). The differences in the number of unmyelinated axons were not statistically significant (p = 0.978). Myelinated axons were counted at 1000× magnification using unbiased stereological methods and Bioquantsoftware. Unmyelinated axons were counted at 5000× magnification using unbiased sampling methods.