Mean ± SEM concentration | ||||||
---|---|---|---|---|---|---|
3 months | 12-15 months | |||||
Tissue-fluid | Aβ1−x | Aβ40 | Aβ42 | Aβ1−x | Aβ40 | Aβ42 |
ISF-hippo (pg/ml) | 727.7 ± 92.1 | 224.4 ± 23.3 | 93.6 ± 16.9 | 626.4 ± 93.9 | 345.9 ± 52.2 | 73.9 ± 38.9 |
ISF-striatum (pg/ml) | 207.1 ± 31.8 | 142.1 ± 19.5 | ||||
CSF (pg/ml) | 11337 ± 699.7 | 5058 ± 496.0 | 2375 ± 336.8 | 8451 ± 915.3 | 4272 ± 898.7 | 961 ± 170.0 |
Hippo-carbonate (pg/mg protein) | 255.0 ± 42.9 | 70.1 ± 14.5 | 80.3 ± 15.4 | 1147.6 ± 383.7 | 93.2 ± 29.7 | 692.5 ± 194.9 |
Hippo-guanidine (pg/mg protein) | 1126 ± 135.9 | 105 ± 21.7 | 291 ± 30.4 | 87476 ± 29714 | 8159 ± 2775 | 66214 ± 22315 |
The concentration of ISF eAβ was assessed by microdialysis (n = 21). CSF was isolated from the cisterna magna and directly analyzed by ELISA (n = 6). Hippocampal tissues were processed by sequential extraction in carbonate buffer and then 5 m guanidine (n = 6). Tissue Aβ values are normalized to protein concentrations for each sample. Hippo, Hippocampus.