Table 1.

Changes in gene expression in sodium butyrate-treated R6/2 mice

Brain region GenBank accession number Probe ID mRNA Direction of change Percentage of change calls
Striatum plus cortex X61940 104598_at MKP-1 Increase 100
Striatum plus cortex V00714 94781_at Hemoglobin, α adult chain 1 Increase 100
Striatum plus cortex J00413 101869_s_at Hemoglobin, β adult major chain Increase 100
Cortex V00722 103534_at Hemoglobin, β 1 Increase 75
Cortex V00727 160901_at c-fos oncogene Increase 100
Cortex AI854404 160131_at Angiomotin like 2 Increase 100
Cortex AI845584 93285_at MAPK phosphatase-3 (MKP-3) Increase 75
Cortex M88354 93381_at Arginine vasopressin Increase 75
Striatum D45859 101836_at Protein phosphatase 1B, β isoform Increase 75
Striatum AB028272 96254_at DNAJ-heat shock protein 40 Increase 75
Striatum plus cortex C79248 94689_at Unknown Decrease 75
Cortex L31397 103031_g_at Dynamin Decrease 75
Cortex M18775 102431_at Microtubule-associated protein tau Decrease 75
Cortex AV330064 161114_i_at Unknown Decrease 75
Striatum AJ007909 98525_f_at Erythroid differentiation regulator Decrease 100
Striatum AI451558 97161_at Unknown Decrease 75
Striatum AI153421 96215_f_at Unknown Decrease 75
Striatum D00073 95350_at Transthyretin Decrease 75
Striatum AA711516 96302_at Unknown Decrease 75
Striatum AI183202 92724_at Heterogeneous nuclear ribonucleoprotein A1 Decrease 75

  • Samples from sodium butyrate-treated R6/2, untreated R6/2, and wild-type mice (n = 4 from each group) were analyzed for differential mRNA expression by microarray. Array data are expressed as increased or decreased based on a minimum difference call cutoff of three of four independent comparisons using samples from individual mice (see Materials and Methods). Most important in the gene array findings was that three known genes (α-and β-globin and MKP-1) were highly increased in expression. These increases in mRNAs were specific to both the striatum and neocortex and were detected in all eight array comparisons.