Summary of distinct regions of Olig2 and Nkx2.2 essential for nuclear localization, physical interaction, and cross-repression
| Olig2 | S/T residues | bHLH domain | Ala-rich region |
|---|---|---|---|
| Promoting MN formation | - | + | - |
| Physical interaction with Nkx2.2 | - | + | - |
| Cross-repression of Nkx2.2 | - | + | ± |
| Induction of Sox10 | + | + | + |
| Nkx2.2 | N terminus | Homeodomain | NK-2-specific domain |
|---|---|---|---|
| Transporting Olig2 into nuclei | - | + | + |
| Physical interaction with Olig2 | - | + | - |
| Induction of Sox10 | + | + | + |
Nkx2.2 and Olig2 protein structure analysis narrows down domains that are essential for their physical interaction and cross-repression in regulating neuronal subtype acquisition. The + indicates domains that are necessary for these interactions; the - indicates regions that are not essential for these interactions. The bHLH domain of Olig2 and the homeodomain of Nkx2.2 are the core regions for their structural and functional interactions.