Table 1.

Distributions of immunogold label on cleft and cytoplasmic sides of isolated PSDs

Diameter (nm) Number Total label (gold/0.1 μm2) Distribution (%)
PSD-95
Cleft 374 ± 23 37 35 ± 2.1 47
Cytoplasmic 335 ± 15 39 37 ± 3.0 53
CaMKII
Cleft 293 ± 11 57 3.9 ± 0.7 12
Cytoplasmic 358 ± 13 45 28.0 ± 3.2 88
Shank
Cleft 301 ± 9.0 88 1.7 ± 0.2 10
Cytoplasmic 288 ± 10 57 16 ± 1.9 90
  • PSDs were labeled with immunogold, and the density ± SEM of gold label was measured in unselected electron micrographs on both cleft and cytoplasmic surfaces of the PSDs. For each antibody, the percentage of label on the cleft and cytoplasmic surfaces was calculated.