Table 1.

Summary of bchs alleles and phenotypes

Genotype	Mean life span	SD	pvalue with WT	BCHS expression	Increase in IUCP^*	Ubiquitin aggregates	APPL aggregates
Df(dsf3)/Df(clot7)bchs¹	18.2 n = 190	6.3	<0.0001	0	22.8	+	+
Df(dsf4)/Df(clot7)bchs²	19.6 n = 136	6.5	<0.0001	0	nd	+	+
bchs³402Cy011/Df(clot7)	19.1 n = 209	5.6	<0.0001	0	16	+	+
bchs⁴EP(2)2299/Df(clot7)	21.7 n = 161	7.9	<0.0001	++/0	6.9	+	+
bchs⁴EP(2)2299/EP(2)2299	22.9 n = 135	8.3	<0.0001	++/0	nd	+	+
bchs⁵/bchs⁵	21.5 n = 99	8.5	0.0002^1-159	nd	nd	+	nd
bchs^rev1/bchs^rev1	27.2 n = 81	7.7	0.81^1-159	nd	nd	0	nd
Wild-type (Canton S)	32 n = 123	9.1	1	++++	0	0	0
402CyO/Df(clot7) genotype control	32.5 n = 151	5.8	0.67	++++	nd	0	0
dsf1/Df(clot7) regional control	31.5 n = 84	8.3	0.62	+++	nd	0	0

↵* Insoluble ubiquitin conjugated proteins.
The mean life span for each genotype is indicated, as are SDs andp value (Student t Test). Comparisons with wild-type (Canton S) and
↵F1-159 note that pvalues were calculated from wild-type controls aged at the same time and under the same conditions (average = 27.6, SD = 11.9, andn = 73). The + and 0 symbols used for BCHS expression summarize the Northern and Western analyses of the message and/or protein. The +/0 symbols used forbchs⁴ allele reflect the presence of the protein in young adults and its absence in aged individuals. The relative levels of ubiquitin and APPL aggregate formation detected by confocal (starting at 10 d of age) are summarized by + (presence) or 0 (absence) symbols. Of the three independent experiments done to examine levels of insoluble ubiquitin-conjugated proteins (IUCP), the representative Western blot in Figure 6 was used to calculate the fold increase after correcting for load (histone 2B) and normalization to wild-type controls.