CRE-dependent expression of luciferase
| Type of culture | Treatment | Luciferase activity (RLU) |
|---|---|---|
| Neuron-enriched | Control | 16.5 ± 1.4 (18)6-150 |
| CGRP (2 × 300 nm) | 42.9 ± 6.0 (18)6-150,6-160 | |
| Non-neuronal | Control | 1.3 ± 0.5 (18) |
| CGRP (2 × 300 nm) | 3.5 ± 1.0 (17) |
Cultures were treated 24 hr after transfection with CRE-luciferase DNA. Multiple treatments with CGRP occurred at 2 hr intervals. Luciferase activity was measured approximately 21 hr after initiation of treatment with CGRP and is reported as RLU per 1000 per well. Numbers in parentheses are numbers of wells per treatment (five or six wells per treatment per experiment, three experiments per treatment). One value was deleted from the CGRP-treated group of non-neuronal cells because it was >2 SDs of the mean for the group.
↵F6-150 p < 0.001 compared with non-neuronal cultures of the same treatment.
↵F6-160 p < 0.001 compared with control within the same type of culture (two-way ANOVA with Tukey's multiple-comparisons test).