Confirmation of select gene changes by quantitative RT-PCR
| RT-PCR | Microarray | ||||
|---|---|---|---|---|---|
| UniGene | Gene | Ratio | p value | Ratio | p value |
| Hippocampus, chronic ECS | |||||
| Rn.964 | Insulin-like growth factor II (somatomedin A) | 0.33 | 4.4E-02 | 0.44 | 5E-02 |
| Rn.11400 | Prostaglandin D synthase | 0.34 | 3.9E-02 | 0.35 | 6E-03 |
| Rn.19727 | Hairy and enhancer of split 1 (Drosophila) | 1.27 | 1.8E-02 | 2.09 | 5E-03 |
| Rn.18568 | Rat smallest neurofilament protein (NF-L) mRNA | 1.31 | 2.1E-03 | 1.22 | 4E-02 |
| Rn.7044 | Glypican 1 | 1.33 | 1.6E-02 | 1.28 | 3E-04 |
| Rn.78188 | Heme oxygenase-3 (H0-3) mRNA | 1.42 | 2.3E-02 | 1.61 | 9E-03 |
| Rn.859 | Ras homolog enriched in brain | 1.54 | 6.7E-04 | 1.45 | 3E-03 |
| Rn.9756 | Protein phosphatase 1, regulatory (inhibitor) subunit 1A | 1.58 | 4.8E-06 | 1.62 | 6E-03 |
| Rn.3546 | Neuritin | 1.58 | 8.6E-05 | 1.44 | 8E-04 |
| Rn.31799 | Pyruvate dehydrogenase phosphatase isoenzyme 1 | 1.72 | 1.3E-03 | 1.83 | 2E-02 |
| Rn.9714 | Neuropeptide Y | 2.40 | 5.2E-06 | 2.14 | 1E-02 |
| Rn.22 | Thyrotropin releasing hormone | 21.42 | 1.8E-03 | 2.01 | 1E-02 |
| Hippocampus, acute ECS | |||||
| Rn.87787 | Glutamate receptor, metabotropic 1 | 0.63 | 2.5E-06 | 0.60 | 3E-05 |
| Rn.19727 | Hairy and enhancer of split 1 (Drosophila) | 0.71 | 3.3E-02 | 1.55 | 4E-05 |
| Rn.3812 | Normalized rat heart, Bento Soares Rattus sp. cDNA RHEAE07 | 0.72 | 1.4E-05 | 0.65 | 2E-03 |
| Rn.9113 | Aldehyde dehydrogenase family 3, subfamily A2 | 0.86 | 5.6E-03 | 0.44 | 2E-05 |
| Rn.3360 | Growth factor receptor bound protein 2 | 1.36 | 3.1E-02 | 1.68 | 6E-06 |
| Rn.10815 | Neuropilin | 1.36 | 1.2E-04 | 1.67 | 2E-03 |
| Rn.9797 | Fibroblast growth factor receptor 1 | 1.44 | 1.6E-03 | 1.51 | 2E-03 |
| Rn.2045 | Protein tyrosine phosphatase type IVA, member 2 | 1.46 | 2.0E-03 | 1.51 | 3E-03 |
| Rn.3546 | Neuritin | 1.59 | 4.9E-06 | 1.56 | 1E-08 |
| Rn.9839 | Nuclear receptor subfamily 4, group A, member 2 | 1.76 | 6.8E-05 | 1.70 | 1E-05 |
| Rn.44320 | Avian sarcoma virus 17 (v-jun) oncogene homolog | 1.80 | 1.7E-07 | 1.84 | 9E-05 |
| Rn.859 | Ras homolog enriched in brain | 1.83 | 1.0E-07 | 1.42 | 1E-05 |
| Rn.11183 | DNA-damage inducible transcript 3 | 1.89 | 1.6E-08 | 1.78 | 4E-03 |
| Rn.3723 | IFN-related developmental regulator 1 | 1.93 | 1.3E-07 | 2.01 | 3E-05 |
| D82074 | Rattus sp. mRNA for BHF-1 | 1.97 | 7.0E-04 | 0.44 | 6E-04 |
| S65091 | cAMP-regulated phosphoprotein | 2.52 | 6.7E-05 | 2.09 | 7E-07 |
| Rn.2398 | TGFB inducible early growth response | 2.79 | 1.4E-08 | 3.62 | 1E-06 |
| Rn.40517 | Activity and neurotransmitter-induced early gene protein 4 (ania-4) | 3.64 | 2.9E-10 | 2.58 | 6E-07 |
| Rn.44369 | Prostaglandin-endoperoxide synthase 2 | 4.03 | 1.5E-07 | 7.33 | 9E-08 |
| Rn.25754 | Tissue inhibitor of metalloproteinase 1 | 4.71 | 8.7E-08 | 11.39 | 2E-06 |
| Rn.11266 | Brain-derived neurotrophic factor | 5.73 | 2.9E-08 | 3.85 | 2E-04 |
| S82649 | Neuronal activity-regulated pentraxin | 7.24 | 2.0E-10 | 5.89 | 7E-09 |
| Rn.37500 | Homer, neuronal immediate early gene, 1 | 7.42 | 2.5E-07 | 6.50 | 2E-07 |
| Frontal cortex, chronic ECS | |||||
| Rn.11400 | Prostaglandin D synthase | 0.61 | 1.0E-02 | 0.71 | 0.03 |
| Rn.7730 | Prostaglandin E synthase | 2.18 | 1.5E-02 | not on U34A chip | |
| Rn.506 | Synapsin 2 | 1.29 | 1.0E-03 | 1.34 | 0.0004 |
| Frontal cortex, acute ECS | |||||
| Rn.32777 | Ceruloplasmin (ferroxidase) | 1.35 | 2.8E-02 | 1.81 | 2E-05 |
| Rn.10599 | Phosphoinositide 3-kinase, regulatory subunit, polypeptide 1 | 1.59 | 5.8E-04 | 2.02 | 4E-03 |
| Rn.6977 | Lost on transformation 1 | 1.90 | 2.1E-04 | 1.58 | 8E-05 |
| Rn.1716 | Interleukin-6 receptor | 2.24 | 3.1E-08 | 1.74 | 3E-05 |
All samples for ECS-treated groups and sham control groups used for microarray studies were analyzed for each gene (n = 8 - 10 per group).