Bmax values of GR and MR cytosolic binding sites specifically labeled by [3H]corticosterone in the hippocampus and the cerebral cortex of GR-i mice compared with WT mice
| [3H]Corticosterone specifically bound, Bmax (fmol/mg protein) | ||||
|---|---|---|---|---|
| GR | MR | |||
| WT (n = 3) | GR-I (n = 4) | WT (n = 3) | GR-I (n = 4) | |
| Hippocampus | 29.4 ± 3.5 | 14.4 ± 2.7*(-51%) | 11.0 ± 1.4 | 12.0 ± 3.6(ns) |
| Cerebral cortex | 11.8 ± 1.7 | 6.0 ± 1.3*(-49%) | 4.5 ± 1.4 | 6.3 ± 2.3(ns) |
Bmax is expressed as femtomoles [3H]corticosterone specifically bound per milligram of protein. Each value is the mean ± SEM of n independent determinations. ns, Nonsignificant; *p<0.05, compared with respective values in WT mice (unpaired Student's t test).