MyHC isoform mRNAs in mystacial pads
| Proportion of MyHC isoform mRNAs (%) | ||||
|---|---|---|---|---|
| MyHC1 | MyHC2A | MyHC2B | MyHC2D | |
| Mouse | nd | nd | 89.5 ± 3.54 | 10.5 ± 3.54 |
| Rat | nd | nd | 100 | nd |
| Guinea pig | ? | ? | + | ? |
Total RNA was extracted from whole mystacial pads of mouse, rat, and guinea pig and analyzed by RT-PCR. The fluorescence intensities of the ethidium bromide-stained PCR products as observed on agarose gel electrophoresis were used to compare the MyHC isoform mRNA levels in different species. nd, Not detected; ?, no appropriate primers for these mRNAs were available.