Quantification of the phosphorylation level of MBS
| Relative fluorescence intensity (cell number) | ||
|---|---|---|
| Genotype | MBS | Phosphorylated MBS |
| RhoA DN | ||
| Wild type | 1.00 ± 0.05 (525) | 1.00 ± 0.01 (466) |
| Cre+/RhoA DN+ | 0.98 ± 0.05 (480) | 0.69 ± 0.03** (472) |
| Rho-K DN | ||
| Wild type | 1.00 ± 0.05 (486) | 1.00 ± 0.01 (476) |
| Cre+/Rho-K DN+ | 1.00 ± 0.07 (511) | 0.79 ± 0.07* (487) |
The sections through the thoracic spinal cord of the wild-type and double-transgenic embryos at E10.5 were stained with anti-lsl1 Ab and either anti-MBS or antiphosphorylated MBS Ab. The fluorescence intensity of MBS or phosphorylated MBS signals in single lsl1-positive cells was measured, and the relative fluorescence intensity was calculated as the ratio of the fluorescence intensity relative to the average of the intensity in the wild-type embryos. Data are the mean ± SEM (n = 4 for each genotype). The number of cells used for measurement of the fluorescence intensity is shown in parentheses. Asterisks indicate p values according to Student's t test; *p < 0.01, **p < 0.001 versus wild-type mice.