The role of BDNF in regulating filopodia number on retinal growth cones
| Treatment | Filopodia (n) |
|---|---|
| Chick | |
| No treatment | 6.3 ± 0.3 (102) |
| BDNF | 10.3 ± 0.3 (152)* |
| NGF | 6.3 ± 0.3 (74) |
| NT-3 | 6.2 ± 0.3 (62) |
| Control IgG | 6.2 ± 0.2 (80) |
| CHEX | 8.0 ± 0.3 (80)* |
| K252a | 6.76 ± 0.3 (80) |
| K252a plus BDNF | 8.66 ± 0.3 (80)* |
| Mouse | |
| p75+/+ no treatment | 7.1 ± 0.8 (85) |
| p75+/+ BDNF | 9.6 ± 1.2 (81) |
| p75-/- no treatment | 9.8 ± 0.5 (56)** |
| p75-/- BDNF | 10.5 ± 0.7 (69) |
Cultured retinal explants were treated with 160 nM BDNF, NGF, or NT-3 for 30 min or 1:100 CHEX for 4 hr. Cultures were pretreated with 1 μm K252a for 1 hr followed by addition of 160 nM BDNF for 30 min. Number of filopodia was determined for each growth cone. n denotes number of growth cones analyzed to determine filopodial number. *p<0.001 indicates statistical significance relative to controls; two-sample t test. **p<0.005 denotes statistical significance between p75-/- no treatment versus p75+/+ no treatment; two-sample t test.