Decreased hippocampal cell number in Fgfr1 conditional null mice
| Embryonic and perinatal mice | ||||||
|---|---|---|---|---|---|---|
| Total number of cresyl violet-stained cells (× 103) | ||||||
| Hippocampal VZ | Hippocampal DG | |||||
| Genotype | E16.5 | P0 | P7 | E16.5 | P0 | P7 |
| Fgfr1flox/flox | 156.5 | 377.9 | 452.9 | 152.5 | 598.5 | 637.6 |
| hGFAP-Cre/+; Fgfr1flox/flox | 189.2 | 178.2 | 304.4 | 131.1 | 381.5 | 224.0 |
| Percentage decrease | — | 52.9 | 32.8 | 14.1 | 36.3 | 64.9 |
| Adult mice | ||||
|---|---|---|---|---|
| Total number of immunostained cells (× 103) | ||||
| Hippocampal CA fields | Hippocampal DG | |||
| Genotype | NeuN | PV | NeuN | PV |
| Fgfr1flox/flox | 697 ± 74.8 | 26.0 ± 1.6 | 614.2 ± 57.6 | 5.94 ± 1.16 |
| hGFAP-Cre/+; Fgfr1flox/flox | 339 ± 26.5*** | 13.0 ± 1.0*** | 437.3 ± 14.6* | 2.09 ± 0.57* |
| Percentage decrease | 51.4 | 50.0 | 28.8 | 64.8 |
Values were obtained by unbiased stereological analyses in serial sections stained as indicated. For cresyl violet staining at the three perinatal ages shown, a total of six mice per genotype was statistically analyzed by ANOVA, which showed an overall significant effect of genotype on cell number (p < 0.001). For the NeuN-and parvalbumin (PV)-stained sections of adult mice, three brains per genotype were analyzed. ANOVA showed an overall significant effect of genotype on NeuN cell number (F = 29.0; p < 0.001) and on PV cell number (F = 54; p < 0.0001). Post hoc Sheffe tests showed that control mice significantly differed from mutants in both regions (*p < 0.05; **p < 0.01; ***p < 0.001).