Table 1.

Effects of ethanol, progesterone, CB34, and GHB on 3α, 5α-THP concentrations in isolated rat hippocampal minces after a 30 min incubation

Control
Treatment ng/gm−1 tissue Percentage of change
Vehicle 0.50 ± 0.04
Ethanol
25 mm 0.56 ± 0.03 13 ± 6.1
50 mm 0.85 ± 0.04* 70 ± 8.3*
100 mm 0.96 ± 0.02* 92 ± 5.0*
Progesterone (1 μm) 1.05 ± 0.03* 110 ± 7.1*
CB34 (30 μm) 1.22 ± 0.04* 145 ± 7.8*
GHB (300 μm) 1.37 ± 0.04* 175 ± 8*

  • Minces of freshly isolated rat hippocampus (see Materials and Methods) were incubated for 30 min in the presence of the different drugs or vehicle. Data are expressed as ng/gm−1 tissue ± SEM obtained from hippocampal minces of eight rats per group of treatment and also as percentage of change ± SEM from the value measured in vehicle-treated minces. *p < 0.01 versus vehicle.