Protein | Rab3ABCD−/− | Rab3A+/+ −BCD−/− | Antibody |
---|---|---|---|
β-Actin | 99.4 ± 1.9 | 100 ± 2.3 | Actin |
α-Synuclein | 97.7 ± 6.1 | 100 ± 3.9 | Syf |
α-Tubulin | 101.3 ± 0.8 | 100 ± 1.1 | Tub |
β-Synuclein | 102.4 ± 9.9 | 100 ± 15.9 | U1128 |
CAPS I | 94.9 ± 6.7 | 100 ± 6.8 | CAPS |
CSP | 102.9 ± 5.8 | 100 ± 6.7 | CSP |
GDI | 101.2 ± 3.2 | 100 ± 1.6 | CI81.2 |
Mint | 101 ± 3.5 | 100 ± 3.4 | P932 |
Munc13-1 | 108.9 ± 4.2 | 100 ± 2.5 | 40 |
Munc13-2 | 100.9 ± 9.2 | 100 ± 6.3 | 44 |
Munc18 | 102.8 ± 3.1 | 100 ± 1.2 | I370 |
Neuroligin I | 103.2 ± 2.8 | 100 ± 2.4 | 4C12 |
Rab3A | 0.5 ± 0.1 | 100 ± 3.6 | CI42.2 |
Rab5 | 102.4 ± 1.9 | 100 ± 1.6 | CI621.3 |
Rabaptin | 100.9 ± 4.6 | 100 ± 2.2 | R321 |
Rabphilin | 56.6 ± 4.4 | 100 ± 2.7 | I734 |
RIM1 | 105.9 ± 3.5 | 100 ± 8.3 | RIM |
Rim1α | 99.3 ± 8.1 | 100 ± 2.4 | U952 |
Rim2β | 96.1 ± 12.7 | 100 ± 3.3 | U952 |
SCAMP1 | 98.9 ± 5.3 | 100 ± 1.9 | P936 |
SCAMP5 | 110.3 ± 7.7 | 100 ± 7.5 | SCAMP5 |
SGT2 | 108.1 ± 6.5 | 100 ± 5.1 | SGT2 |
SNAP-25 | 101.3 ± 0.7 | 100 ± 1.5 | CI71.2 |
Synapsin I | 102.7 ± 2.2 | 100 ± 2.2 | E028 |
Synaptobrevin 2 | 98.2 ± 1.3 | 100 ± 0.7 | CI69.1 |
Synaptogyrin | 100.5 ± 2.2 | 100 ± 3.9 | P925 |
Synaptophysin 1 | 105.7 ± 5.1 | 100 ± 4 | 580 |
Synaptotagmin 1 | 100 ± 3.3 | 100 ± 3.9 | V216 |
Proteins in the brains of newborn mice (postnatal day 1) were measured by quantitative immunoblotting using internal standards and 125I-labeled secondary antibodies and phosphorimager detection as described previously (Rosahl et al., 1995; Schlüter et al., 1999). CAPS, Calcium-activated protein of secretion; CSP, cystein string protein; GDI, GDP-dissociation inhibitor; SCAMP, secretory carrier membrane protein 1; SGT2, small glutamine-rich tetratri-copeptide repeat-containing protein; SNAP, soluble N-ethylmaleimide-sensitive factor attached protein.