The rate of NADH and H2O2 generation by α-KGDH at different NADH/NADH plus NAD+ ratios
| NADH/NAD+ plus NADH | NADH generation (pmol/sec) | H2O2 generation (pmol/sec) | NADH generation per H2O2 generation |
|---|---|---|---|
| 0 | 46 ± 2.8 | 0.15 ± 0.02 | 307 |
| 0.2 | 29.2 ± 2.3 | 2.58 ± 0.11 | 11.4 |
| 0.5 | 8.0 ± 0.19 | 17.8 ± 1.3 | 0.45 |
Experiments were performed as described for Figure 3, a and c, but NADH plus NAD+ was applied in different ratios at 500 μm NADH plus NAD+ concentration.
Values are mean ± SEM from three experiments.