E9.5 VZ | E12.5 VZ | E14.5 VZ | E16.5 VZ | E18.5 VZ | E16.5 SVZ | E18.5 SVZ | |
---|---|---|---|---|---|---|---|
Rostral | |||||||
+/+ | 43.0 | 49.5 | 34.0 | 42.7 | 42.7 | 20.1 | 36.8 |
SEM | 1.9 | 2.3 | 2.3 | 1.8 | 3.5 | 2.9 | 2.0 |
-/- | 48.1 | 55.2 | 40.8 | 42.5 | 44.2 | 19.9 | 25.7 |
SEM | 1.8 | 1.2 | 1.5 | 2.0 | 2.9 | 2.4 | 2.8 |
p value | 0.98 | 0.001 | |||||
Intermediate | |||||||
+/+ | 43.2 | 39.2 | 49.0 | 39.2 | 20.8 | 31.1 | |
SEM | 2.1 | 1.8 | 3.0 | 5.1 | 2.7 | 2.2 | |
-/- | 47.1 | 44.1 | 42.2 | 40.3 | 17.9 | 26.0 | |
SEM | 1.6 | 1.4 | 2.4 | 2.5 | 2.3 | 2.2 | |
p value | 0.46 | 0.1 | |||||
Caudal | |||||||
+/+ | 52.8 | 42.8 | 38.1 | 44.6 | 46.8 | 18.2 | 30.5 |
SEM | 1.3 | 1.5 | 1.5 | 2.2 | 3.1 | 2.0 | 1.4 |
-/- | 55.5 | 49.3 | 45.2 | 42.3 | 38.2 | 15.9 | 24.1 |
SEM | 1.2 | 1.2 | 1.9 | 2.3 | 2.3 | 1.8 | 1.8 |
p value | 0.10 | 0.0015 | 0.0032 | 0.38862 | 0.0014 | 0.242 | 0.0043 |
There was a significant decrease in the LI of caudal PCs only. Two-sample t test values for the arsine square root transformed data are shown. The MANCOVA indicated that the change in the LI over time was significantly different between wild-type and mutant animals in the caudal regions only. Although the SVZ is seeded with fewer cells at E16.5, their cell cycle is not significantly different from normal. By E18.5, the LI in SVZ PCs is decreased.