Wild type | High-OE myo/NT-3 | Wild type NT-3 treated | ||||
---|---|---|---|---|---|---|
Contralateral | Ipsilateral | Contralateral | Ipsilateral | Contralateral | Ipsilateral | |
Lamina I-II | ||||||
Saline | 1.40 ± 0.23 | 3.60 ± 1.27 | 3.60 ± 1.60 | 4.13 ± 2.53 | ||
Acid | 1.73 ± 0.64 | 14.87 ± 1.05* | 3.07 ± 1.88 | 3.07 ± 1.20** | 1.0 ± 0.32 | 1.2 ± 0.49 |
Lamina III-IV | ||||||
Saline | 3.07 ± 0.84 | 3.60 ± 0.53 | 9.40 ± 5.20 | 9.73 ± 5.25 | ||
Acid | 5.27 ± 0.74 | 12.93 ± 1.73* | 7.87 ± 3.99 | 8.27 ± 2.97 | 1.2 ± 0.37 | 1.4 ± 0.51 |
Lamina V-VI | ||||||
Saline | 8.07 ± 1.13 | 7.93 ± 0.88 | 21.33 ± 12.08 | 22.60 ± 10.60 | ||
Acid | 12.53 ± 1.27 | 23.00 ± 1.21* | 15.93 ± 7.53 | 17.87 ± 5.97 | 1.0 ± 0.55 | 0.6 ± 0.24 |
The number of Fos-positive cells was counted from different spinal laminas from both ipsilateral and contralateral sides of the spinal cord 5 d after acid injection. Comparisons were made between groups using one-way ANOVA and Fisher's PLSD. In regard to NT-3 overexpression, wild-type mice were derived from litters within the myo/NT-3 colony. For exogenous treatments, wild-type CF-1 mice were purchased from Charles River. In wild-type mice, acid injection caused significant increases in the number of Fos-positive neurons compared with saline-injected mice in each lamina (*p < 0.05). In contrast, acid treatment did not increase Fos-positive neurons in high-OE myo/NT-3 as well as in wild-type mice treated with NT-3. Moreover, the number of Fos-positive nuclei was significantly lower in acid-injected high-OE myo/NT-3 mice compared with acid-injected wild-type mice (**p < 0.05). Data are plotted as means ± SEM; n = 3 animals per group.