Quantification of pyknotic nuclei in the VB on thionine-stained coronal sections from control wild-type (+/+) and control NMDAR1 knock-out (−/−) neonates, as well as animals treated with MK-801 or PCP (0.5 or 8 mg/kg, s.c., respectively, to dams starting 24 hr before birth at t = 0,8, and 16 hr)
| PO controls | PO MK-801 | PO PCP | ||||
|---|---|---|---|---|---|---|
| Mice:VB | ||||||
| I | 4:6 | 3:6 | 2:4 | 4:6 | 2:4 | 4:7 |
| II | 4:7 | 4:8 | 4:7 | 4:8 | 3:5 | 4:7 |
| III | 4:8 | 4:8 | 4:6 | 4:8 | 3:6 | 4:8 |
| IV | 3:5 | 3:4 | 4:6 | 4:5 | 3:4 | 4:8 |
| Pyknotic nuclei/mm2 ± SD | ||||||
| I | 394 ± 147 | 1547 ± 451 | 1065 ± 405 | 799 ± 412 | 505 ± 282 | 486 ± 160 |
| II | 337 ± 173 | 1302 ± 714 | 666 ± 523 | 1005 ± 714 | 738 ± 554 | 963 ± 505 |
| III | 106 ± 83 | 974 ± 225 | 397 ± 232 | 941 ± 734 | 509 ± 416 | 1118 ± 544 |
| IV | 30 ± 13 | 120 ± 79 | 95 ± 46 | 71 ± 62 | 30 ± 18 | 51 ± 15 |
Four anatomically matched rostrocaudal levels were analyzed (I-IV). Levels are as in Figure 1C.