Fits to distributions of shut time durations
| Gly(μm) | τ1 (ms) | τ2 (ms) | τ3 (ms) | τ4 (ms) | |
|---|---|---|---|---|---|
| (n) | Area (%) | Area (%) | Area (%) | Area (%) | |
| 10 | 0.04 ± 0.01 | 0.3 ± 0.1 | 1.7 ± 0.3 | — | Within bursts |
| (4) | (33 ± 5%) | (60 ± 2%) | (8 ± 2%) | ||
| 50 | 0.03 ± 0.03 | 0.21 ± 0.02 | 0.9 ± 0.2 | 43 ± 10 | Within clusters |
| (5) | (37 ± 6%) | (46 ± 1%) | (13 ± 5%) | (4 ± 1%) | |
| 100 | 0.04 ± 0.01 | 0.21 ± 0.01 | 1.1 ± 0.2 | 37 ± 5 | Within clusters |
| (6) | (51 ± 2%) | (42 ± 6%) | (3 ± 1%) | (3 ± 1%) | |
| 200 | 0.04 ± 0.01 | 0.20 ± 0.10 | 1.8 ± 0.1 | — | Within clusters |
| (2) | (52 ± 2%) | (44 ± 6%) | (3 ± 1%) | ||
| 500 | 0.03 ± 0.01 | 0.16 ± 0.02 | 1.1 ± 0.2 | — | Within clusters |
| (4) | (64 ± 6%) | (32 ± 6%) | (4 ± 1%) | ||
| 1000 | 0.03 ± 0.01 | 0.10 ± 0.01 | 0.50 ± 0.03 | — | Within clusters |
| (5) | (65 ± 2%) | (34 ± 2%) | (1 ± 0.1%) |
Shut-times distribution were fitted with three or four exponential components. The values of the time constants are similar at different concentrations. However, in the experiments at 50 and 100 μm, an extra slow component (∼40 ms) was detected. This component represented long-lived shut states observed within clusters.