Quantification of colocalization with Pearson correlation coefficients
| Wild type | P37A | K38A | K38A/R40A | |
|---|---|---|---|---|
| GABARAP/γ2 | 0.691 ± 0.018*** | 0.407 ± 0.050 | 0.495 ± 0.028* | 0.371 ± 0.036 |
| GABARAP/NSF | 0.775 ± 0.011*** | 0.496 ± 0.051* | NT | 0.559 ± 0.064 |
The colocalization of the mutant forms of YFP-GABARAP is altered with respect to the γ2 subunit immunofluorescence staining and the NSF immunofluo-rescence staining in cultured hippocampal neurons. The Pearson correlation coefficient measures the strength and direction of a relationship between two variables, generating values from -1 (a perfect negative correlation) to +1 (a perfect positive correlation), with zero being a random correlation. The significance of a Pearson correlation is measured using Student's t test. NT, Not tested. *p < 0.05; ***p < 0.001.