Primer pairs designed for RT-PCR
| Primers | Sequence (5′ → 3′) | Annealing temperature | RT-PCR product size |
|---|---|---|---|
| Actin F1 | ATATCGCTGCGCTGGTCGTC | 55°C | 753 bp |
| Actin R1 | ACCGCTCGTTGCCAATAGTGAT | ||
| Calretinin F3 | TTGGCGGAAGTATGACACAGAC | 51°C | 302 bp |
| Calretinin R3 | CAATATAGCCGCTTCCATCCTT | ||
| Reelin F2 | ACTCTGGCAACCGTACAATAATG | 55°C | 343 bp |
| Reelin R2 | GACCGGATCAGCAGAAGAACTA | ||
| SCNFUA | TTCATCGGCGTCATCAT | 51°C | 565 bp |
| SCNRUA | ACATCATCAGAGCAAAGAGC | ||
| SCNFUB | GGAGACATTCCTCCAGAGAT | 51°C | 556 bp (SCN1/SCN2) or 560 bp (SCN3) |
| SCNRUB | ATCATGACGTCAGACAGCTT |
SCNFUB, SCNRUB, SCNFUA, and SCNRUA are universal SCN primers designed in short regions of strict identity between cDNA MSCN1a, MSCN2a, and MSCN3a. F, Forward equal direct sense primer; R, reverse.