Current relaxations after depolarizing voltage steps
| τfast (ms) | Afast (%) | τslow1 (ms) | Aslow1(%) | τslow2 (ms) | Aslow2 (%) | n | |
|---|---|---|---|---|---|---|---|
| Agonists plus 0 blocker | 0.338 ± 0.016 | 88.5 ± 1.6 | 14.5 ± 6.9 | 11.5 ± 1.6 | 3 | ||
| Agonists plus 100 μm amantadine | 0.279 ± 0.027 | 53.1 ± 3.3 | 5.17 ± 0.51 | 27.5 ± 1.9 | 153. ± 6.0 | 19.4 ± 1.6 | 4 |
| Agonists plus 100 μm Mg2+ | 0.399 ± 0.031 | 71.3 ± 1.5 | 8.51 ± 0.42 | 28.7 ± 1.5 | 3 |
The membrane voltage of HEK 293T cells expressing NR1/NR2B receptors was stepped from −67 to 40 mV in the presence of agonists (30 μm NMDA plus 10 μm glycine) with 0 blocker, 100 μm amantadine, or 100 μm Mg2+. Current relaxations at 40 mV were fit using either a double (agonists with 0 blocker or 100 μm Mg2+) or a triple (agonists with 100 μm amantadine) exponential equation. A is the fractional amplitude of each component.