Binding affinity of Amino-Nogo fragments to NgR
| Amino acid number | Amino acid sequence | NgR Kd (nm) |
|---|---|---|
| 181-864 (fragment X) | No binding at 150 nm | |
| 622-1018 (fragment Y) | 6.66 ± 1.49 | |
| 877-1018 (fragment Y4) | 9.01 ± 6.36 | |
| 950-1018 (fragment Y4C) | 3.51 ± 3.36 | |
| 971-1018 | 2.69 ± 1.32 | |
| 995-1018 (Nogo-A-24) | IFSAELSKTSVVDLLYWRDIKKTG | 2.43 ± 0.51 |
| 995-1015 | IFSAELSKTSVVDLLYWRDIK | 4.55 ± 3.66 |
| 995-1014 | IFSAELSKTSVVDLLYWRDI | 3.19 ± 0.12 |
| 995-1013 | IFSAELSKTSVVDLLYWRD | 2.48 ± 0.72 |
| 996-1018 | FSAELSKTSVVDLLYWRDIKKTG | 26.59 ± 6.86 |
| 1000-1018 | LSKTSVVDLLYWRDIKKTG | No binding at 25 nm |
| 1005-1018 | VVDLLYWRDIKKTG | No binding at 25 nm |
| 950-1004 | .........IFSAELSKTS | No binding at 400 nm |
| Amino of Nogo-C | MDGQKKNWKDKVVDLLYWRDIKKTG | No binding at 400 nm |
| Amino of Nogo-B | No binding at 50 nm |
Binding Kd values for AP-fused Amino-Nogo fragments were measured by applying conditioned media containing the AP fusion protein to NgR-expressing COS-7 cells. Bound AP was stained and measured.