Primer sequences used for the specific amplification of CA enzymes in adrenal gland medulla cDNA
| CA | Forward primer sequence | Reverse primer sequence |
|---|---|---|
| CAI | 5′-AAACCAGCGAAGCCAAAC-3′ | 5′-TGTGGTGGACGGTGGTTG-3′ |
| CAII | 5′-CCGACAGTCCCCTGTGGACAT-3′ | 5′-GCGGAGTGGTCAGAGAGCCA-3′ |
| CAIII | 5′-GCTTGCCTCCTCCTGGTG-3′ | 5′-GCAGGGTGGCGTGGTGAA-3′ |
| CAIV | 5′-AGTCCCCCATCAACATCG-3′ | 5′-AGGCAGGGGCAAGGGTAG-3′ |
| CAVII | 5′-AACGGAGCGAGCGGACAG-3′ | 5′-TCGCTGAGTGGGGGTGTG-3′ |
| CAIX | 5′-CCGTTTCCCTGCCGAGAT-3′ | 5′-AGGACAGGACAGTTACCG-3′ |
| CAXII | 5′-TCTGATTTCCTTCTGCTA-3′ | 5′-AGAGGGAGGAGTAGAAGG-3′ |