Mutant | Founder | Germ-line transmission | RT-PCR | Immunostaining in wild-type mice | Crossed with Gjb 1-null mice |
---|---|---|---|---|---|
C280G | 15 | 12 | 6 (7M15, 7M20, 7M33, 7M37, 7M39, 7M45) | 6 (7M15*, 7M20, 7M33, 7M37, 7M39, 7M45) | 3 (7M33, 7M37, 7M39) |
S281x | 16 | 10 | 4 (8M25, 8M34, 8M35#, 8M39) | 3 (8M25, 8M34*, 8M39*) | 1 (8M25) |
Approximately one-half of the transgenic founders with germ-line transmission in wild-type background (the numbers of lines are indicated) were screened by immunohistochemistry and RT-PCR, thereby identifying lines that expressed Cx32 in all myelin sheaths and human Cx32 mRNA (typically at levels that were higher than endogenous/mouse mRNA), respectively. We excluded three lines (*) that showed non-uniform expression by immunostaining and one line (#) in which there was less human/mutant Cx32 than endogenous mouse Cx32 mRNA. The male transgenic (hemizygous) mice from some of these lines were bred with Gjb 1-null female mice; the resulting male offspring were used for additional analysis.