Primary antibodies used in this study
| Antibody | Immunogen | Host | Specificity | Application | Source |
|---|---|---|---|---|---|
| 6E10 | aa 1-17 of Aβ | Mouse | H, M | IHC, IF, WB | Signet (Dedham, MA) |
| CT20 | aa 751-770 of human APP | Rabbit | H, M | WB | Calbiochem |
| GFAP | Bovine GFAP | Rabbit | H, M, R | IHC, IF | DAKO (Carpinteria, CA) |
| CD11b | Mouse B-cells | Rat | M | IHC | Serotec (Raleigh, NC) |
| CD45 | Mouse B-cells | Rat | M | IHC | Serotec |
| Iba1 | C terminus of Iba1 | Rabbit | H, M, R | IF | WAKO (Richmond, VA) |
| HT7 | aa 159-163 of tau | Mouse | H | IHC, WB | Innogenetics (Alpharetta, GA) |
| AT8 | Peptides containing phospho-S202/T205 | Mouse | H, M, R | IHC, IF, WB | Innogenetics |
| AT180 | Peptides containing phospho-T231/S235 | Mouse | H, M, R | IHC, WB | Innogenetics |
| PHF1 | Peptides containing phospho-S396/S404 | Mouse | H, M, R | IHC, WB | a |
| β-Actin | C terminus of actin | Rabbit | Wide range | WB | Sigma |
| Aβ40 | aa 35-40 of Aβ | Mouse | H, M, R, D | ELISA | Mayo Clinic (Jacksonville, FL) |
| Aβ42 | aa 35-42 of Aβ | Mouse | H, M, R, D | WB | Mayo Clinic |
| p35/p25 | C terminus of human p35 | Rabbit | H, M, R | WB | Santa Cruz Biotechnology (Santa Cruz, CA) |
| cdk5 | aa 16 of human cdk5 | Rabbit | H, M, R | WB | Calbiochem |
| JNK | Human JNK2 | Rabbit | H, M, R | WB | Cell Signaling (Beverly, MA) |
| Phospho-JNK | Phosphopeptides around The183/Tyr185 | Mouse | H, M, R | WB | Cell Signaling |
| GSK-3β | N terminus of rat GSK-3β | Mouse | H, M, R, D, C | WB | BD Transduction (San Diego, CA) |
| Phospho-GSK-3β | Phosphopeptides around Tyr216 | Mouse | H, M, R, D | WB | BD Transduction |
| Phospho-GSK-3β | Phosphopeptides around Ser9 | Rabbit | H, M, R | WB | Cell Signaling |
| p38 | Human p38 | Rabbit | H, M, R | WB | Cell Signaling |
| Phospho-p38 | Phosphopeptides around Thr180/Tyr182 | Rabbit | H, M, R | WB | Cell Signaling |
H, Human; M, mouse; R, rat; D, dog; C, chicken; IHC, immunohistochemistry; IF, immunofluorescent staining; WB, Western blotting.
↵ a Generous gift from P. Davis (Albert Einstein College of Medicine, Bronx, NY).